Below are the season-opening assignments for Appalachian League alumni in affiliated ball.
| Name | Position | Appy League Team | MLB Team | Level | Affiliate | League |
|---|---|---|---|---|---|---|
| Nate Ackenhausen | LHP | Bluefield Ridge Runners | Kansas City Royals | High-A | Quad Cities River Bandits | Midwest |
| Chase Adkison | C | Elizabethton River Riders | St. Louis Cardinals | High-A | Peoria Cheifs | Midwest |
| Tayler Aguilar | OF | Greeneville Flyboys | Colorado Rockies | Rookie | ACL Rockies | ACL |
| Maui Ahuna | SS | Burlington Sock Puppets | San Francisco Giants | Single-A | San Jose Giants | California |
| Trevor Austin | SS | Greeneville Flyboys | Houston Astros | High-A | Asheville Tourists | South Atlantic |
| Brett Banks | RHP | Johnson City Doughboys | New York Mets | High-A | Brooklyn Cyclones | South Atlantic |
| Jake Bockenstedt | RHP | Johnson City Doughboys | Chicago White Sox | High-A | Winston-Salem Dash | South Atlantic |
| Ethan Bosacker | RHP | Johnson City Doughboys | Kansas City Royals | Double-A | Northwest Arkansas Naturals | Texas |
| Sam Brodersen | RHP | Bluefield Ridge Runners | St. Louis Cardinals | Single-A | Palm Beach Cardinals | Florida State |
| Canyon Brown | C | Two Teams | Kansas City Royals | High-A | Quad Cities River Bandits | Midwest |
| Marcus Brown | SS | Elizabethton River Riders | Washington Nationals | High-A | Wilmington Blue Rocks | South Atlantic |
| Homer Bush Jr. | OF | Greeneville Flyboys | Tampa Bay Rays | Double-A | Montgomery Biscuits | Southern |
| Caleb Cali | 3B | Danville Otterbots | Seattle Mariners | Double-A | Arkansas Travelers | Texas |
| Brendan Collins | RHP | Burlington Sock Puppets | Washington Nationals | High-A | Wilmington Blue Rocks | South Atlantic |
| Drew Conover | RHP | Princeton WhistlePigs | Athletics | High-A | Lansing Lugnuts | Midwest |
| Jack Crowder | RHP | Kingsport Axmen | Baltimore Orioles | Unassigned | -- | Unassigned |
| Kaelen Culpepper | SS | Bluefield Ridge Runners | Minnesota Twins | High-A | Cedar Rapids Kernels | Midwest |
| Dylan Cumming | RHP | Burlington Sock Puppets | San Francisco Giants | Double-A | Richmond Flying Squirrels | Eastern |
| Brady Day | SS | Princeton WhistlePigs | Philadelphia Phillies | Single-A | Clearwater Threshers | Florida State |
| Brandon Decker | RHP | Bristol State Liners | New York Yankees | Single-A | Tampa Tarpons | Florida State |
| Joel Dragoo | OF | Greeneville Flyboys | Philadelphia Phillies | Single-A | Clearwater Threshers | Florida State |
| Connor Dykstra | C | Danville Otterbots | Seattle Mariners | Single-A | Modesto Nuts | California |
| Daniel Eagen | RHP | Greeneville Flyboys | Arizona Diamondbacks | High-A | Hillsboro Hops | Northwest |
| Magnus Ellerts | RHP | Danville Otterbots | Cleveland Guardians | Double-A | Akron RubberDucks | Eastern |
| Alan Espinal | C | Johnson City Doughboys | Colorado Rockies | Single-A | Fresno Grizzlies | California |
| Donye Evans | RHP | Johnson City Doughboys | Detroit Tigers | Single-A | Lakeland Flying Tigers | Florida State |
| Caleb Farmer | C | Burlington Sock Puppets | Washington Nationals | High-A | Wilmington Blue Rocks | South Atlantic |
| Jackson Feltner | 1B | Bluefield Ridge Runners | Arizona Diamondbacks | High-A | Hillsboro Hops | Northwest |
| Hunter Fitz-Gerald | 3B | Kingsport Axmen | Seattle Mariners | Double-A | Arkansas Travelers | Texas |
| Ty Floyd | RHP | Bristol State Liners | Cincinnati Reds | Single-A | Daytona Tortugas | Florida State |
| Connor Foley | RHP | Johnson City Doughboys | Arizona Diamondbacks | Single-A | Visalia Rawhide | California |
| TJ Fondtain | LHP | Pulaski River Turtles | Tampa Bay Rays | High-A | Bowling Green Hot Rods | South Atlantic |
| Zach Fruit | RHP | Greeneville Flyboys | Baltimore Orioles | Double-A | Chesapeake Baysox | Eastern |
| Will Gagnon | RHP | Burlington Sock Puppets | Los Angeles Dodgers | Rookie | ACL Dodgers | ACL |
| Andres Galan | RHP | Princeton WhistlePigs | Tampa Bay Rays | Rookie | FCL Rays | FCL |
| Travis Garnett | LHP | Johnson City Doughboys | Arizona Diamondbacks | Single-A | Visalia Rawhide | California |
| Paul Gervase | RHP | Bristol State Liners | Tampa Bay Rays | Triple-A | Durham Bulls | International |
| Dub Gleed | 3B | Greeneville Flyboys | Miami Marlins | Unassigned | -- | Unassigned |
| Gunner Gouldsmith | SS | Kingsport Axmen | Athletics | Single-A | Stockton Ports | California |
| Riley Gowens | RHP | Danville Otterbots | Chicago White Sox | Double-A | Birmingham Barons | Southern |
| Rodney Green Jr. | OF | Pulaski River Turtles | Athletics | High-A | Lansing Lugnuts | Midwest |
| Woody Hadeen | SS | Princeton WhistlePigs | Detroit Tigers | Single-A | Lakeland Flying Tigers | Florida State |
| Bryson Hammer | LHP | Burlington Sock Puppets | Colorado Rockies | High-A | Spokane Indians | Northwest |
| Tyler Hampu | RHP | Princeton WhistlePigs | Colorado Rockies | Single-A | Fresno Grizzlies | California |
| Caleb Hobson | OF | Two Teams | Colorado Rockies | High-A | Spokane Indians | Northwest |
| Garrett Hodges | RHP | Danville Otterbots | Milwaukee Brewers | Single-A | Carolina Mudcats | Carolina |
| Carson Jacobs | RHP | Greeneville Flyboys | Chicago White Sox | High-A | Winston-Salem Dash | South Atlantic |
| Jacob Jenkins-Cowart | 1B | Burlington Sock Puppets | Miami Marlins | Single-A | Jupiter Hammerheads | Florida State |
| Brayden Jobert | 1B | Bluefield Ridge Runners | St. Louis Cardinals | High-A | Peoria Chiefs | Midwest |
| Marques Johnson | RHP | Johnson City Doughboys | San Francisco Giants | High-A | Eugene Emeralds | Northwest |
| Ryan Johnson | 3B | Pulaski River Turtles | Houston Astros | Double-A | Corpus Christi Hooks | Texas |
| Brendan Jones | OF | Elizabethton River Riders | New York Yankees | High-A | Hudson Valley Renegades | South Atlantic |
| Kyle Karros | 3B | Greeneville Flyboys | Colorado Rockies | Double-A | Hartford Yard Goats | Eastern |
| Cameron Leary | OF | Pulaski River Turtles | Athletics | Single-A | Stockton Ports | California |
| Jordan Leasure | RHP | Elizabethton River Riders | Chicago White Sox | MLB | Chicago White Sox | MLB |
| Carlos Lequerica | RHP | Pulaski River Turtles | Detroit Tigers | High-A | West Michigan Whitecaps | Midwest |
| Andrew Lindsey | RHP | Kingsport Axmen | Tampa Bay Rays | Single-A | Charleston RiverDogs | Carolina |
| Jared Lyons | RHP | Danville Otterbots | Cincinnati Reds | Double-A | Chattanooga Lookouts | Southern |
| Aiden May | RHP | Kingsport Axmen | Miami Marlins | Single-A | Jupiter Hammerheads | Florida State |
| Bryce Mayer | RHP | Greeneville Flyboys | Houston Astros | Single-A | Fayetteville Woodpeckers | Carolina |
| Alex McCoy | OF | Elizabethton River Riders | San Diego Padres | Unassigned | -- | Unassigned |
| Ryan McCrystal | C | Burlington Sock Puppets | Cincinnati Reds | Single-A | Daytona Tortugas | Florida State |
| Haydn McGeary | 1B | Bluefield Ridge Runners | Chicago Cubs | Double-A | Knoxville Smokies | Southern |
| Xavier Meachem | RHP | Burlington Sock Puppets | Miami Marlins | High-A | Beloit Sky Carp | Midwest |
| Noah Mendlinger | OF | Bristol State Liners | St. Louis Cardinals | Double-A | Springfield Cardinals | Texas |
| Eddie Micheletti Jr. | OF | Greeneville Flyboys | Toronto Blue Jays | High-A | Vancouver Canadians | Northwest |
| Andrew Morones | RHP | Greeneville Flyboys | Kansas City Royals | High-A | Quad Cities River Bandits | Midwest |
| Callan Moss | 1B | Two Teams | Kansas City Royals | High-A | Quad Cities River Bandits | Midwest |
| Kole Myers | OF | Burlington Sock Puppets | Los Angeles Dodgers | High-A | Great Lakes Loons | Midwest |
| Braden Nett | RHP | Greeneville Flyboys | San Diego Padres | Double-A | San Antonio Missions | Texas |
| Steven Ondina | SS | Johnson City Doughboys | Baltimore Orioles | Single-A | Delmarva Shorebirds | Carolina |
| Brennan Orf | 1B | Elizabethton River Riders | Toronto Blue Jays | High-A | Vancouver Canadians | Northwest |
| Deniel Ortiz | 3B | Kingsport Axmen | St. Louis Cardinals | Single-A | Palm Beach Cardinals | Florida State |
| Avery Owusu-Asiedu | OF | Elizabethton River Riders | Philadelphia Phillies | Single-A | Clearwater Threshers | Florida State |
| Darius Perry | C | Greeneville Flyboys | Colorado Rockies | High-A | Spokane Indians | Northwest |
| Sam Petersen | OF | Kingsport Axmen | Washington Nationals | Single-A | Fredericksburg Nationals | Carolina |
| Ryan Picollo | OF | Burlington Sock Puppets | Seattle Mariners | Single-A | Modesto Nuts | California |
| Zimo 'Zack' Qin | LHP | Burlington Sock Puppets | San Diego Padres | Single-A | Lake Elsinore Storm | California |
| Luke Russo | RHP | Greeneville Flyboys | Philadelphia Phillies | High-A | Jersey Shore BlueClaws | South Atlantic |
| Ryan Schiefer | RHP | Greeneville Flyboys | Tampa Bay Rays | Single-A | Charleston RiverDogs | Carolina |
| Jack Seppings | RHP | Princeton WhistlePigs | Milwaukee Brewers | Single-A | Carolina Mudcats | Carolina |
| D'Andre Smith | 2B | Burlington Sock Puppets | New York Mets | High-A | Brooklyn Cyclones | South Atlantic |
| Caswell Smith | RHP | Greeneville Flyboys | Arizona Diamondbacks | Single-A | Visalia Rawhide | California |
| Myles Smith | OF | Greeneville Flyboys | Cincinnati Reds | High-A | Dayton Dragons | Midwest |
| Jack Snyder | RHP | Danville Otterbots | Tampa Bay Rays | High-A | Bowling Green Hot Rods | South Atlantic |
| Austin St. Laurent | INF | Burlington Sock Puppets | Seattle Mariners | Single-A | Modesto Nuts | California |
| Adam Stone | RHP | Danville Otterbots | New York Yankees | Single-A | Tampa Tarpons | Florida State |
| Jacob Stretch | RHP | Princeton WhistlePigs | Baltimore Orioles | Unassigned | -- | Unassigned |
| Will Taylor | OF | Greeneville Flyboys | Pittsburgh Pirates | Single-A | Bradenton Marauders | Florida State |
| Kavares Tears | OF | Kingsport Axmen | San Diego Padres | Rookie | ACL Padres | ACL |
| Zach Thornton | LHP | Elizabethton River Riders | New York Mets | High-A | Brooklyn Cyclones | South Atlantic |
| Danny Trehey | RHP | Princeton WhistlePigs | Houston Astros | Single-A | Fayetteville Woodpeckers | Carolina |
| Austin Troesser | RHP | Greeneville Flyboys | New York Mets | High-A | Brooklyn Cyclones | South Atlantic |
| Cody Tucker | RHP | Danville Otterbots | San Francisco Giants | High-A | Eugene Emeralds | Northwest |
| Fidel Ulloa | RHP | Bristol State Liners | Colorado Rockies | Single-A | Fresno Grizzlies | California |
| Joe Vetrano | 1B | Johnson City Doughboys | Los Angeles Dodgers | High-A | Great Lakes Loons | Midwest |
| Cameron Wagoner | RHP | Greeneville Flyboys | Milwaukee Brewers | High-A | Wisconsin Timber Rattlers | Midwest |
| Mario Zabala | RHP | Elizabethton River Riders | San Diego Padres | Single-A | Lake Elsinore Storm | California |